FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16107812
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC197650.081.521454120
GAGCACA317250.050.72311120
AGAGCAC427800.037.608166118-119
AAGAGCA646800.025.565588120
GAAGAGC752400.022.668839120
GGAAGAG921600.018.680088120
CGGAAGA1092300.015.719891118-119
TCGGAAG1099950.015.298415118-119
ATCGGAA1269600.013.560167116-117
GATCGGA1307900.012.940334116-117
AGATCGG1442300.012.006374114-115
TAGATCG369450.010.898895114-115
CACACGT55550.010.73826120
CTAGATC91100.010.051101112-113
GAGATCG536050.09.608359112-113
TTAGATC127300.09.209257110-111
AAGATCG449400.09.183398120
CCTAGAT32500.09.070715114-115
CAGATCG496850.08.760856112-113
ATAGATC163550.08.270698112-113
Produced by FastQC (version 0.11.5)