FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14351901
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC142850.075.353836120
GAGCACA241200.044.41145120
AGAGCAC322900.034.914124120
AAGAGCA509150.021.949907120
GAAGAGC557650.020.49768120
GGAAGAG667250.017.189543120
TCGGAAG798950.015.181711120
CGGAAGA787950.015.020622120
ATCGGAA953550.013.432676120
GATCGGA954300.013.114115120
TAGATCG243850.012.214354120
AGATCGG1067400.011.797582118-119
CTAGATC60750.010.267631118-119
GAGATCG391500.09.042654120
AAGATCG381300.08.984387116-117
TTAGATC98250.08.874867118-119
CAGATCG395850.08.662775120
ACAGATC192200.08.190154120
ATAGATC131150.07.918761120
CCTAGAT26450.07.9022846120
Produced by FastQC (version 0.11.5)