FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13243626
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC143450.071.5815120
GAGCACA236950.043.1934120
AGAGCAC304400.033.72333118-119
AAGAGCA485700.021.065868118-119
GAAGAGC534850.019.538721120
GGAAGAG641350.016.367739120
CGGAAGA719800.015.46374120
TCGGAAG721100.014.917845118-119
ATCGGAA848000.012.927793118-119
GATCGGA859950.012.857358120
AGATCGG943850.011.97144120
TAGATCG202700.011.019588116-117
CTAGATC53600.010.056822116-117
AAGATCG462800.09.485358118-119
GAGATCG290900.09.102937118-119
TTAGATC93450.09.012876118-119
CAGATCG259850.08.751525118-119
ACGTCTG59900.07.5364065120
TAAGATC207550.07.4013047108-109
CACGTCT50150.07.1206765120
Produced by FastQC (version 0.11.5)