FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16991291
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG31200.024.229086120
TCCGATC70450.017.3718473
CCGATCT61600.017.335494
GCGTCGG74300.015.5036335120
AAAAAAA6561650.015.448701120
CGGTGGG28550.014.710011120
CGATCTA19150.010.6514055
CTTCCGA127900.09.9550081
AAAGAGG197600.09.594476120
CATTAAA1967050.09.583229120
TCATTAA2090250.09.348473120
ATCATTA2281950.09.165208120
ATTAAAA2061000.08.814526120
GTATCAT2582000.08.734483120
TATCATT2534700.08.601604120
CCGTATC2958050.08.559098120
CGTATCA2728550.08.434664120
TTAAAAA1992800.08.384604120
GCCGTAT3168200.08.176946120
TAAAAAA2026350.08.139194120
Produced by FastQC (version 0.11.5)