FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16991291
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC243400.0201.70451120
GAGCACA414350.0110.89033120
AGAGCAC572000.083.528076118-119
AAGAGCA844450.054.374977118-119
GAAGAGC943600.050.542427116-117
GGAAGAG1139750.040.255337116-117
CGGAAGA1355900.035.234314114-115
TCGGAAG1391450.033.032642114-115
ATCGGAA1608350.029.857975112-113
GATCGGA1610550.028.61665112-113
AGATCGG1727300.027.702332110-111
CTAGATC97300.024.8956110-111
CACGTCT65050.024.412775118-119
TAGATCG417350.024.060326110-111
GAGATCG543800.023.382673110-111
ACACGTC62450.023.243809120
CACACGT56750.022.792658120
AAGATCG594750.022.696907110-111
TTAGATC163650.021.785685108-109
CAGATCG463950.021.736622110-111
Produced by FastQC (version 0.11.5)