FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11898335
Sequences flagged as poor quality0
Sequence length52-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT161800.010.941651120
GTGTAGA240850.010.589651120
AGTGTAG238050.010.58816120
AAGAGTG331900.010.541458120
AGAGTGT292450.010.3833685120
GAGTGTA259400.010.2950735120
AGATCTC173700.09.639261120
TAGGGAA528700.09.568803120
AGGGAAA572000.08.970352120
GTAGGGA569550.08.945718120
TGTAGAT257150.08.891558120
GTAGATC253100.08.559619120
GGAAAGA524850.08.55275120
AAAGAGT392250.08.4606085120
GTGTAGG678300.08.4050665120
ATCTCGG176900.08.379314120
CGTGTAG725050.08.376294120
TGTAGGG645350.08.350652120
GAAAGAG467950.08.335915120
TCGTGTA770550.08.201002120
Produced by FastQC (version 0.11.5)