FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11898335
Sequences flagged as poor quality0
Sequence length50-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC143650.052.650394120
GAGCACA200850.039.589626120
AGAGCAC262900.030.100336120
AAGAGCA384100.021.779198120
GAAGAGC432400.019.493647120
GGAAGAG518400.017.168526120
TCGGAAG606650.014.83864118-119
CGGAAGA614400.014.734629120
ATCGGAA739800.013.192243120
ACACGTC45450.012.326494120
GATCGGA768100.012.299829118-119
CACGTCT39400.011.310782120
CACACGT39400.011.229787118-119
AGATCGG881750.010.945526118-119
TAGATCG185150.010.728073120
CTAGATC51250.09.689281120
TTAGATC69250.08.687385116-117
AAGATCG377500.08.668361120
GCTAGAT25850.08.127261120
GAGATCG333100.07.9316635120
Produced by FastQC (version 0.11.5)