FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11393744
Sequences flagged as poor quality0
Sequence length58-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA491900.08.733542120
GCGTCGG68100.08.017676120
GGTCGCC261300.07.9219832120
AGATCTC449800.07.896898120
TAGATCT466850.07.8912406120
CTCGGTG363550.07.6743755120
GATCTCG441000.07.673522120
ATCTCGG414600.07.6556244120
GTGGTCG279750.07.5925455120
TCTCGGT383450.07.495162120
TGGTCGC263150.07.4105496118-119
TCGGTGG357500.07.4014497120
GGTGGTC303750.07.3679523120
GTAGATC591400.07.233732120
AGTGTAG612050.07.146524120
CGGTGGT353100.07.1028523120
AGAGTGT695400.07.100998120
GTGTAGA599750.07.0930037120
GAGTGTA651150.06.883254120
TGTAGAT611700.06.826923120
Produced by FastQC (version 0.11.5)