FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11393744
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC125650.066.71254120
GAGCACA172700.043.637836120
AGAGCAC237100.034.796356120
AAGAGCA366300.022.9382120
GAAGAGC418200.019.823162118-119
GGAAGAG545900.016.457243120
TCGGAAG646500.014.059986120
CGGAAGA656300.013.406838120
GATCGGA763700.012.724632120
ATCGGAA764700.011.982061118-119
CACGTCT49650.011.583792120
AGATCGG839500.011.17432118-119
CTAGATC44200.010.918561116-117
TAGATCG174850.010.888738120
ACACGTC56350.09.38527120
TTAGATC71850.09.016749120
AAGATCG382000.08.661748116-117
GAGATCG275450.08.592107118-119
ACGTCTG70350.08.26931120
CACACGT43450.08.215892120
Produced by FastQC (version 0.11.5)