FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11861585
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC156800.086.477165120
GAGCACA247800.052.395992120
AGAGCAC325400.040.864765118-119
AAGAGCA484850.027.118217120
GAAGAGC562700.023.830502116-117
GGAAGAG687500.019.091463118-119
CGGAAGA797950.016.931417114-115
TCGGAAG807050.016.105684116-117
ATCGGAA945450.014.247005112-113
CGATCTA19950.013.8301775
GATCGGA946550.013.708407114-115
AGATCGG1032250.013.00564110-111
TAGATCG280200.012.091454112-113
TCCGATC72300.011.9065783
CCGATCT70700.011.7857754
CTAGATC57300.010.833144114-115
TTAGATC79700.09.8998575118-119
GAGATCG341250.09.709195110-111
AAGATCG337300.09.600694114-115
ACACGTC46800.09.428787120