FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12080127
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1176400.013.325846120
TCCGATC60350.013.124413
CCGATCT60250.012.64823154
GCGTCGG71700.012.053689120
CTTCCGA95900.010.6366831
GCCGTAT620350.09.974669120
CGCCGTA665550.09.838314120
CCGTATC589950.09.695146120
CGTATCA520750.09.577416120
TGGTCGC896850.09.301899120
GTGGTCG965550.09.255428120
GGTCGCC838200.09.115015120
CATTAAA397300.09.003355120
GTATCAT511000.08.949746120
ATCATTA470600.08.9146120
GTCGCCG792600.08.912489120
TCATTAA412100.08.811086120
TCGCCGT747650.08.6536045120
GGTGGTC1037550.08.60737120
CGGTGGT1147150.08.438995120
Produced by FastQC (version 0.11.5)