FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16088041
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC180750.077.37554120
GAGCACA283600.050.982628120
AGAGCAC390200.038.21412120
AAGAGCA602850.024.659067118-119
GAAGAGC693400.021.247335120
GGAAGAG847850.018.007484120
CGGAAGA1008550.015.287761120
TCGGAAG1015250.015.036887118-119
ATCGGAA1178850.012.883295118-119
GATCGGA1190600.012.850808116-117
AGATCGG1299500.011.684337116-117
TAGATCG346500.010.955221114-115
CTAGATC72750.010.539205114-115
GCCTAGA11153.4851837E-910.452639120
GAGATCG518750.09.250873114-115
TTAGATC121500.09.016493112-113
AAGATCG418250.08.42935114-115
CAGATCG430250.08.267203112-113
CCTAGAT27000.07.8612547112-113
ATAGATC175850.07.669069116-117
Produced by FastQC (version 0.11.5)