FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12936961
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC187300.0135.45232120
GAGCACA303750.079.919365120
AGAGCAC407050.061.052494118-119
AAGAGCA604300.040.19051118-119
GAAGAGC669400.036.982117116-117
GGAAGAG817650.029.766094116-117
CGGAAGA949450.026.049849114-115
TCGGAAG976950.024.744745114-115
CACACGT46500.024.027586120
CACGTCT53000.023.848993120
ATCGGAA1146550.021.796797112-113
GATCGGA1177600.020.675833112-113
AGATCGG1262900.019.769808110-111
ACACGTC57250.019.121595120
TAGATCG321900.018.316963110-111
ACGTCTG71150.017.130764120
GAGATCG474150.015.506316112-113
GCACACG69150.015.504555120
CTAGATC58700.015.1888485114-115
CAGATCG479200.015.059545110-111
Produced by FastQC (version 0.11.5)