FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14563610
Sequences flagged as poor quality0
Sequence length50-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC225100.099.442474120
GAGCACA378550.059.70653120
AGAGCAC516150.044.94382120
AAGAGCA748750.030.88519120
GAAGAGC897400.025.957483118-119
GGAAGAG1053750.021.908031116-117
CGGAAGA1228100.018.958136116-117
TCGGAAG1260300.018.310698120
ATCGGAA1450850.016.075012114-115
GATCGGA1527800.015.110307112-113
AGATCGG1692900.013.741117112-113
TAGATCG323900.013.051125110-111
CACACGT42700.012.8228245120
CTAGATC70400.012.10077112-113
GAGATCG786900.010.876852110-111
ACTAGAT23850.010.803494120
GCTAGAT25900.010.705225114-115
CAGATCG724100.010.621571118-119
TTAGATC63700.010.47165114-115
TCTAGAT21900.010.294768120
Produced by FastQC (version 0.11.5)