FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12377628
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC164250.083.820984120
GAGCACA271150.050.69394120
AGAGCAC369000.038.697567120
AAGAGCA540350.026.24354118-119
GAAGAGC629000.022.306534118-119
GGAAGAG739900.018.998508116-117
CGGAAGA872100.016.17237120
TCGGAAG892950.015.741991114-115
ATCGGAA1022550.013.893405114-115
GATCGGA1046900.013.531808112-113
AGATCGG1159650.012.294956112-113
CTAGATC60700.011.5031185108-109
CCTAGAT21150.011.408048120
TAGATCG248450.011.329991120
TTAGATC88000.010.344252110-111
GAGATCG446350.010.156298112-113
AAGATCG365500.010.122402114-115
CAGATCG373850.09.778943112-113
GCTAGAT23550.09.624357108-109
CACACGT52100.09.613037118-119
Produced by FastQC (version 0.11.5)