FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16583369
Sequences flagged as poor quality0
Sequence length52-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG533050.08.665909120
TAGATCT395750.08.534513120
GAGTGTA570250.08.468801120
AGAGTGT624850.08.256843120
AGATCTC409100.08.006718120
GTGTAGA526450.07.9540734120
AAGAGTG695750.07.906922120
GTAGATC529050.07.8809648120
ATCTCGG367650.07.734551120
TGGTCGC218900.07.7284904120
GTGGTCG244800.07.7195225120
TAGGGAA993950.07.671359120
TCTCGGT329800.07.585369120
TGTAGAT569600.07.572691120
TGTAGGG1104800.07.4500947120
GTAGGGA1034850.07.40295120
CGTGTAG1267950.07.347856120
GTGTAGG1198400.07.3387737120
GGGAAAG988750.07.165635120
GATCTCG406300.07.0578494120
Produced by FastQC (version 0.11.5)