FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12458121
Sequences flagged as poor quality0
Sequence length55-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG248200.010.249032120
GAGTGTA278100.09.794306120
GTGTAGA250200.09.615588120
AAGAGTG351750.09.363905120
TAGATCT176550.09.311113120
AGAGTGT309150.09.19873120
GTAGGGA537700.09.004348120
AGATCTC182200.08.627236120
TAGGGAA513500.08.377172120
GTGTAGG635350.08.290873120
TGTAGGG577100.08.233659120
GTAGATC258100.08.112528120
CGTGTAG674350.08.0604925120
ATCTCGG174300.07.9512124120
TCGTGTA723150.07.8732963120
GGGAAAG515600.07.7728868120
GATCTCG193000.07.77144120
GTCGTGT766000.07.714827120
TCTCGGT158500.07.5704117120
CGTCGTG838150.07.4014635120
Produced by FastQC (version 0.11.5)