FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12407117
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC134800.067.50551120
GAGCACA206600.041.450645120
AGAGCAC273150.032.569305118-119
AAGAGCA419100.022.852913120
GAAGAGC487600.019.854366120
GGAAGAG586500.016.616491120
CGGAAGA691800.014.292648120
TCGGAAG691300.014.134825120
ATCGGAA811850.012.879207120
GATCGGA827950.012.636564120
AGATCGG932050.011.578581120
TAGATCG221150.010.746795118-119
CTAGATC59650.010.340232116-117
GATCCTA22000.09.3365621
TTAGATC76550.09.111642118-119
GAGATCG433750.08.591211120
CAGATCG336450.08.062076120
AAGATCG308450.07.8517156118-119
GATCCTG102550.07.7829621
CCCTAGA7205.773818E-77.6248198110-111
Produced by FastQC (version 0.11.5)