FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13690785
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC189800.095.654724120
GAGCACA303300.057.505665120
AGAGCAC416200.042.726692120
AAGAGCA621250.029.248747120
GAAGAGC724100.024.897552118-119
GGAAGAG891950.020.598137120
CGGAAGA1050950.017.33416120
TCGGAAG1071250.017.02765118-119
ATCGGAA1231900.014.854159118-119
GATCGGA1269500.014.545579120
AGATCGG1387000.013.182031116-117
TAGATCG344700.012.209378114-115
CTAGATC76450.011.879477114-115
TCCGATC70050.010.9286023
TTAGATC112550.010.920836110-111
CAGATCG572400.010.796912112-113
GAGATCG477700.010.784049114-115
GCTAGAT24800.010.636873120
AAGATCG345550.09.864237116-117
CCTAGAT26750.09.681389108-109
Produced by FastQC (version 0.11.5)