FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12004855
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC162800.085.90169120
GAGCACA258900.052.449707120
AGAGCAC359000.040.024048120
AAGAGCA537400.026.925987120
GAAGAGC610350.023.554445118-119
GGAAGAG750350.018.995579118-119
CGGAAGA873150.016.581573116-117
TCGGAAG893400.015.824707116-117
ATCGGAA1052650.013.884742114-115
GATCGGA1073350.013.299824114-115
AGATCGG1167100.012.535263112-113
TAGATCG225150.011.419792116-117
CTAGATC64900.011.383014116-117
AAGATCG548950.010.429398112-113
ACACGTC43650.010.120907120
CACGTCT41750.010.061097120
GAGATCG341400.09.619735110-111
TTAGATC93550.09.561451114-115
CACACGT41250.09.480769120
CAGATCG349700.09.401769110-111
Produced by FastQC (version 0.11.5)