FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15240866
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC183850.069.92685120
GAGCACA311550.043.210846120
AGAGCAC398500.033.747955120
AAGAGCA617950.022.33657118-119
GAAGAGC692300.019.7984118-119
GGAAGAG816750.017.258873120
CGGAAGA904050.015.218299118-119
TCGGAAG934650.015.000694120
ATCGGAA1090350.012.883439118-119
GATCGGA1112300.012.6101675116-117
AGATCGG1224750.011.505807116-117
CACGTCT62800.011.40958120
TAGATCG275600.010.999412120
CACACGT60500.010.932307120
ACACGTC60450.010.257516120
CTAGATC82400.09.65654112-113
AAGATCG462700.09.336068120
GAGATCG412450.09.328535114-115
TTAGATC110800.08.984804118-119
CAGATCG403350.08.89858116-117
Produced by FastQC (version 0.11.5)