FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19532733
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA403950.010.099605120
GAGTGTA193850.09.965822120
TAGGGAA386250.09.910037120
TGTAGGG457300.09.682293120
AGTGTAG176600.09.682271120
AGAGTGT230650.09.546313120
CGTGTAG544150.08.996936120
GTGTAGG512700.08.940321120
TCGTGTA582150.08.91465120
GTCGTGT626900.08.871653120
AAGAGTG284900.08.823577120
GTAGATC214100.08.602899120
GTGTAGA206400.08.429687120
TAGATCT131500.08.349269120
GGGAAAG434250.08.261986120
CGTCGTG752400.07.7586675120
AGCGTCG858200.07.6270986120
GCGTCGT806900.07.55435120
AAAGAGT361050.07.5109377120
AGGGAAA496050.07.389905120
Produced by FastQC (version 0.11.5)