FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12465732
Sequences flagged as poor quality0
Sequence length58-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG81700.012.778458120
AAAAAAA860000.011.595348120
GGTCGCC514500.09.22449120
TGGTCGC538450.08.769616120
GTGGTCG584100.08.731381120
CGTATCA341950.08.650388120
GCCGTAT439300.08.399727120
CCGTATC387800.08.16916120
TCGGTGG729250.08.153583120
CTCGGTG789250.08.043078120
GGTGGTC628100.08.033752120
GTCGCCG535450.07.8886914120
CGGTGGT689400.07.6501303120
ATCTCGG906050.07.622096120
CGCCGTA452600.07.609368120
TCTCGGT841250.07.602971120
GATCTCG949750.07.593577120
AGATCTC1002050.07.44873120
TAGATCT1056450.07.3605120
TCGCCGT522800.07.322112120
Produced by FastQC (version 0.11.5)