FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12465732
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC184100.080.27717120
GAGCACA292400.050.345654120
AGAGCAC387750.038.974285120
AAGAGCA597550.025.460135120
GAAGAGC664500.023.374668120
GGAAGAG788350.019.628986120
CGGAAGA929650.017.307915120
TCGGAAG975000.016.502874120
ATCGGAA1149650.014.329811120
GATCGGA1182500.013.735679118-119
ACACGTC66050.013.052382120
CACGTCT62950.012.659385120
CACACGT60050.012.546886120
AGATCGG1306750.012.518221116-117
TAGATCG304300.011.68936116-117
CTAGATC73200.011.381577120
AAGATCG408000.011.257572120
GAGATCG446500.010.441043114-115
ACGTCTG79150.010.342919120
CAGATCG441550.010.270938120
Produced by FastQC (version 0.11.5)