FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13815900
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC165500.088.47601120
GAGCACA257900.053.54646120
AGAGCAC352750.040.458908118-119
AAGAGCA544050.025.731445118-119
GAAGAGC604800.023.628206116-117
GGAAGAG730250.019.385366120
CGGAAGA867050.016.664232114-115
TCGGAAG874500.016.069687120
ATCGGAA1041400.014.0252695120
GATCGGA1040850.013.7684118-119
AGATCGG1135150.012.707832110-111
TAGATCG256900.011.819465116-117
CTAGATC69700.011.578167112-113
GAGATCG407050.09.858448112-113
TTAGATC100950.09.600286116-117
CAGATCG447350.09.563559116-117
CACACGT39400.09.543733120
AAGATCG363150.09.477492114-115
GCTAGAT30500.08.456511110-111
ACACGTC46900.08.331964120
Produced by FastQC (version 0.11.5)