FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14381268
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141900.066.87287120
GAGCACA213400.043.005077120
AGAGCAC291900.033.93363120
AAGAGCA468250.021.597887120
GAAGAGC496300.020.090595118-119
GGAAGAG613250.016.047504118-119
CGGAAGA709150.014.275311116-117
TCGGAAG699450.014.124282120
ATCGGAA836050.012.454026120
GATCGGA844450.012.183904120
TAGATCG235200.011.164095120
AGATCGG929200.011.139384118-119
CTAGATC55450.09.189967118-119
GAGATCG351500.08.264299116-117
AAGATCG343100.08.259338120
TTAGATC105900.08.002468110-111
CACACGT38550.07.5869565120
CAGATCG318200.07.507468118-119
ACACGTC45100.06.764991118-119
ATAGATC132150.06.6559563116-117
Produced by FastQC (version 0.11.5)