FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14837238
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC266650.0123.814705120
GAGCACA441050.068.70861120
AGAGCAC599400.052.85337118-119
AAGAGCA868550.035.822403120
GAAGAGC993500.031.892698116-117
GGAAGAG1202400.025.570536116-117
CGGAAGA1415500.022.397593114-115
TCGGAAG1480450.020.707485114-115
CACGTCT69950.019.51454120
ATCGGAA1699100.018.808672112-113
ACACGTC74600.018.045763120
GATCGGA1760350.017.503206112-113
AGATCGG1914400.016.685719110-111
CACACGT70400.016.14545118-119
TAGATCG512250.015.165557110-111
CTAGATC151100.014.733933108-109
CAGATCG741750.013.7849655110-111
TTAGATC170200.013.356437108-109
GAGATCG519050.013.238778112-113
AAGATCG524200.013.045992112-113
Produced by FastQC (version 0.11.5)