FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14791982
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC204600.091.96052120
GAGCACA343200.054.97799120
AGAGCAC466300.041.62403120
AAGAGCA697950.028.190985120
GAAGAGC792650.024.591715118-119
GGAAGAG936700.020.956266118-119
CGGAAGA1087350.017.996607116-117
TCGGAAG1128250.017.327337116-117
ATCGGAA1303750.015.078993114-115
GATCGGA1341550.014.6541195114-115
AGATCGG1480100.013.246068112-113
CTAGATC105250.012.773214110-111
TAGATCG355600.012.648404110-111
GCTAGAT38350.011.122757108-109
TTAGATC122400.010.703771108-109
GAGATCG549050.010.695993114-115
AAGATCG422900.010.563304112-113
CAGATCG522750.010.302091118-119
CCTAGAT34800.010.068112112-113
CGCTAGA12450.09.789047108-109
Produced by FastQC (version 0.11.5)