FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15191846
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC161400.070.30687120
GAGCACA251900.043.973946120
AGAGCAC341550.034.788227120
AAGAGCA531400.022.707275120
GAAGAGC600850.020.752413120
GGAAGAG739500.016.808773118-119
TCGGAAG876600.014.540474120
CGGAAGA864700.014.496428120
ATCGGAA1016550.012.548138118-119
GATCGGA1060400.012.28769120
AGATCGG1181350.011.398228120
TAGATCG298050.010.868391120
CTAGATC73950.010.366448118-119
TTAGATC117250.09.035817116-117
AAGATCG408200.08.727576120
CCTAGAT29250.08.661181118-119
CACACGT44950.08.659548120
GAGATCG476450.08.238962120
CACGTCT57800.08.218215120
CAGATCG419250.07.706384118-119
Produced by FastQC (version 0.11.5)