FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15568382
Sequences flagged as poor quality0
Sequence length61-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG203550.09.196172120
TAGATCT154150.08.757145120
TGTAGGG448150.08.568013120
TAGGGAA400800.08.547359120
GTAGGGA420350.08.478107120
GAGTGTA226350.08.402379120
GTGTAGG493750.08.3842745120
AGAGTGT254300.08.139472120
CGTGTAG525550.07.922622120
GTGTAGA220250.07.7634096120
GTAGATC234200.07.4931173118-119
AGATCTC173150.07.449712120
AGCGTCG764600.07.399476120
TCGTGTA562350.07.3721623120
GTCGTGT597850.07.3558908120
AAGAGTG295750.07.282714120
CGTCGTG686600.07.208978120
CGCTAGA12107.0770846E-56.9417057120
GAGCGTC815750.06.8840227120
AAGAGCG903000.06.7238235120
Produced by FastQC (version 0.11.5)