FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15568382
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC115050.050.9188120
GAGCACA167950.034.880665120
AGAGCAC218950.028.226643120
AAGAGCA357350.018.31702120
GAAGAGC427100.015.775129120
GGAAGAG540550.013.74735120
TCGGAAG561550.013.111943120
GATCGGA649300.012.68469120
AGATCGG725800.012.3971615120
ATCGGAA673100.011.490965120
CGGAAGA620700.011.458372118-119
TAGATCG182200.010.791182118-119
CTAGATC44200.010.087507120
TTAGATC69250.08.182291118-119
TCTAGAT19000.08.1481695120
AAGATCG298650.08.09715118-119
CCTAGAT18600.07.990463118-119
GAGATCG286850.07.7933826120
CAGATCG331800.07.5401263120
GCTAGAT21450.07.0731425112-113
Produced by FastQC (version 0.11.5)