FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12448415
Sequences flagged as poor quality0
Sequence length55-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT331150.010.347307120
AGTGTAG442750.010.260141120
GAGTGTA485400.09.778961120
AGATCTC322100.09.557464120
CTCGGTG248200.09.211688120
GATCTCG317400.09.150702120
ATCTCGG277650.09.120755120
AGAGTGT535900.08.924637120
GTGTAGA430400.08.9232645120
TCTCGGT250200.08.586412120
GTAGATC444350.08.494572120
AAGAGTG603500.08.461908120
GTGGTCG165050.08.435065120
TGTAGAT470250.08.409548120
GGGAAAG825100.08.007497120
AGGGAAA902000.07.9834523120
TAGGGAA882150.07.925004120
TCGGTGG219000.07.864186120
GGTGGTC189650.07.8472023120
TGGTCGC152500.07.830676120
Produced by FastQC (version 0.11.5)