FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12017594
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC170500.088.88616120
GAGCACA271750.054.47088120
AGAGCAC358500.041.68694118-119
AAGAGCA526700.027.93349118-119
GAAGAGC582100.025.447475116-117
GGAAGAG702400.020.724306116-117
CTAGCGT5000.020.02498690-91
CGGAAGA826650.018.059845114-115
TCGGAAG821850.017.785357114-115
GCTAGCG6650.015.7576888-89
ATCGGAA976300.015.451462112-113
ACACGTC52250.015.1850395120
GATCGGA994050.014.921825112-113
CACACGT45100.014.735428118-119
CACGTCT47800.014.586744120
AGATCGG1096200.013.797122110-111
TAGATCG198500.013.330833110-111
TGCTAGC7800.013.00106488-89
CTAGATC54200.012.568593120
ACGTCTG62050.011.882644120
Produced by FastQC (version 0.11.5)