FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10928909
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC167000.085.5651120
GAGCACA261150.053.418636120
AGAGCAC352250.041.573433120
AAGAGCA506950.028.411558118-119
GAAGAGC594900.024.284908120
GGAAGAG708800.020.170404118-119
CGGAAGA819950.017.440857116-117
TCGGAAG837350.016.890089116-117
CACGTCT54900.014.795167120
ATCGGAA981300.014.657427114-115
ACACGTC61550.014.477893120
GATCGGA1010950.014.141741112-113
CACACGT51750.013.867111120
AGATCGG1114950.012.942863112-113
ACGTCTG69650.012.907398120
TAGATCG270000.012.164268114-115
CTAGATC89000.011.741285108-109
CGTCTGA78000.011.727849120
TTAGATC94250.010.878067108-109
CAGATCG440400.010.37626112-113
Produced by FastQC (version 0.11.5)