FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12368759
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135050.079.5068120
GAGCACA217300.047.32534120
AGAGCAC299200.036.792107120
AAGAGCA469350.023.944565120
GAAGAGC519500.021.496546118-119
GGAAGAG660600.017.330086120
TCGGAAG809150.014.801116120
CGGAAGA810350.014.290662118-119
GATCGGA968800.012.753354120
ATCGGAA961250.012.452072120
AGATCGG1055650.011.7939005120
CACACGT54950.011.458056120
TAGATCG247500.010.914237120
CTAGATC56150.010.610323120
AAGATCG434250.09.837511120
ACACGTC52350.09.828622120
CACGTCT49850.09.642483120
GAGATCG363850.09.098889118-119
TTAGATC104300.09.087395120
CAGATCG332300.08.617984120
Produced by FastQC (version 0.11.5)