FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13776594
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC148050.053.36102120
GAGCACA200600.037.312996120
AGAGCAC252500.030.850834120
AAGAGCA397700.019.81554120
GAAGAGC428500.019.254025120
GGAAGAG546650.015.685825120
TCGGAAG618300.014.466061120
CGGAAGA649700.013.247786120
GATCGGA753750.012.288139120
ATCGGAA758250.012.146781120
AGATCGG846250.011.351195120
TAGATCG184450.011.182365120
CTAGATC48300.010.445156118-119
TTAGATC70650.09.383959116-117
ACACGTC44300.08.638404120
AAGATCG377250.08.373075120
CACACGT38900.08.313576118-119
CACGTCT44900.08.2340555120
GAGATCG351150.07.351495120
CCTAGAT16900.07.2721114112-113
Produced by FastQC (version 0.11.5)