FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11881107
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121650.074.06493120
GAGCACA193050.047.192352120
AGAGCAC262500.036.390896120
AAGAGCA412350.022.915573118-119
GAAGAGC465250.020.209332118-119
GGAAGAG596650.016.796333120
TCGGAAG683800.014.312939118-119
CGGAAGA701850.013.887409120
ATCGGAA804000.012.281289120
GATCGGA812450.011.997088118-119
AGATCGG875900.011.093641118-119
CTAGATC49250.010.530552116-117
TAGATCG202100.010.496728114-115
GAGATCG341950.09.168235120
CCTAGAT19650.09.024829116-117
AAGATCG367700.08.763112116-117
TTAGATC81400.08.673287116-117
CAGATCG274650.07.39505118-119
TAAGATC152800.07.2044945114-115
GCTAGAT20750.06.935329112-113
Produced by FastQC (version 0.11.5)