FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11221681
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC97550.058.554867120
GAGCACA146400.039.61552120
AGAGCAC196200.032.78437120
AAGAGCA317750.020.046143120
GAAGAGC355500.018.410776120
GGAAGAG458550.014.314297120
TCGGAAG512900.013.994177120
GATCGGA608300.012.19072120
CGGAAGA555600.012.118299120
TAGATCG175050.012.093446120
ATCGGAA610650.011.928417120
AGATCGG673350.011.626918120
CTAGATC45550.09.968991118-119
AAGATCG297800.09.295934120
TTAGATC73850.09.032349118-119
CCTAGAT17000.08.842148120
GAGATCG243200.08.4470625120
TCTAGAT24700.07.607111120
GCTAGAT19600.07.349054110-111
ACTAGAT17700.07.0770483120
Produced by FastQC (version 0.11.5)