FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17537344
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC258000.0108.56847120
GAGCACA441700.061.501938120
AGAGCAC589750.046.935722118-119
AAGAGCA858450.031.430527118-119
GAAGAGC989000.027.941673116-117
GGAAGAG1173050.022.919136116-117
CGGAAGA1366950.020.357765114-115
TCGGAAG1423100.018.869385114-115
ATCGGAA1646200.017.003286112-113
GATCGGA1703550.015.841334112-113
AGATCGG1861850.014.997572110-111
TAGATCG447700.013.104048110-111
CTAGATC105400.012.148385120
GAGATCG746000.012.054574112-113
TTAGATC168200.011.120595108-109
CAGATCG634050.011.074216110-111
AAGATCG522700.010.735464110-111
TGAGATC323050.010.465966120
CACACGT61200.010.119243120
CACGTCT65850.010.103759118-119
Produced by FastQC (version 0.11.5)