FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14270235
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC161700.063.24247120
GAGCACA251850.042.43287120
AGAGCAC334900.032.39425120
AAGAGCA498900.022.17444120
GAAGAGC591700.018.548876118-119
GGAAGAG709450.016.3339120
TCGGAAG812200.014.119931118-119
CGGAAGA826550.013.807997120
ATCGGAA943800.012.401789120
GATCGGA961550.012.321222120
AGATCGG1069250.011.44405120
TAGATCG232250.010.735719118-119
CTAGATC59650.010.001564118-119
TTAGATC86700.09.498866120
AAGATCG430650.08.899183120
GAGATCG395800.08.814418120
CACACGT53950.07.9331007118-119
CACGTCT59050.07.8519344118-119
TAAGATC142800.07.810714118-119
CCTAGAT20850.07.77528116-117
Produced by FastQC (version 0.11.5)