FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12496945
Sequences flagged as poor quality0
Sequence length65-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA627700.09.21453120
TCGGTGG476050.08.192361120
GTGGTCG383550.08.1188345120
TAGATCT643450.07.92597120
TGGTCGC350200.07.89829120
GGTCGCC357950.07.794332120
CTCGGTG512200.07.7664447120
TCTCGGT527850.07.763516120
AGATCTC618550.07.740631120
GGTGGTC409600.07.529246120
AGTGTAG833700.07.506246120
ATCTCGG572400.07.4213333120
GTGTAGA815050.07.1038103120
AGAGTGT939950.07.091819120
TGTAGAT829950.07.0774865120
GATCTCG626000.06.9967575120
GCCGTAT302550.06.960786120
GTATCAT246750.06.930044120
GAGTGTA885450.06.8981404120
CGGTGGT460250.06.896205120
Produced by FastQC (version 0.11.5)