FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12496945
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC124200.076.95478120
GAGCACA199200.046.384872120
AGAGCAC271400.035.465828120
AAGAGCA415750.022.995472118-119
GAAGAGC468250.020.345057116-117
GGAAGAG568850.016.770836116-117
CGGAAGA680000.014.74193120
TCGGAAG670100.014.66699120
ATCGGAA783150.012.75992118-119
GATCGGA800050.012.250852120
AGATCGG880650.011.339547116-117
TAGATCG218750.010.644194118-119
CTAGATC51100.010.192597120
GAGATCG346650.08.780846120
TTAGATC89200.08.300901108-109
AAGATCG351200.08.188732116-117
CCTAGAT20050.07.7555714112-113
CAGATCG323300.07.379923114-115
CACACGT33400.07.290731120
TAAGATC130850.06.8753204120
Produced by FastQC (version 0.11.5)