FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12261305
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC140200.072.68208120
GAGCACA220450.048.0419120
AGAGCAC299200.035.692345120
AAGAGCA462550.024.262497120
GAAGAGC518800.020.977465118-119
GGAAGAG664150.017.02046120
TCGGAAG758300.014.513241118-119
CGGAAGA777750.014.250879116-117
ATCGGAA898050.012.41374114-115
GATCGGA903850.012.2962055120
AGATCGG997500.011.207234112-113
CTAGATC56700.010.603534118-119
TAGATCG204650.010.506363118-119
CACGTCT45150.010.080944120
ACACGTC47600.09.8475065120
CACACGT42600.09.568103120
TTAGATC84150.09.24406108-109
AAGATCG439650.09.186275116-117
CCTAGAT17200.09.084266118-119
TCTAGAT24900.08.4581585112-113
Produced by FastQC (version 0.11.5)