FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16251212
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC136200.054.128857120
GAGCACA202500.037.12113120
AGAGCAC265800.030.339708120
AAGAGCA450750.019.551529120
GAAGAGC486200.018.294174120
GGAAGAG636250.014.128068120
TCGGAAG703000.013.735086120
CGGAAGA786400.011.838496120
GATCGGA835100.011.802189118-119
ATCGGAA863250.011.608004120
AGATCGG895600.011.223829120
TAGATCG196250.010.545438120
CTAGATC51650.010.230275120
AAGATCG448100.08.92813120
TTAGATC94350.08.400537120
GAGATCG313150.07.793951120
CCTAGAT21000.07.488573120
CAGATCG324150.07.088301116-117
TAAGATC189950.06.8550305120
CACGTCT50350.06.6214747120
Produced by FastQC (version 0.11.5)