FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15859742
Sequences flagged as poor quality0
Sequence length61-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT486400.09.171461120
GGTGGTC356100.09.116958120
GCGTCGG101750.09.04035120
CTCGGTG454750.08.990098120
TAGATCT622100.08.891107120
TCGGTGG427350.08.849018120
ATCTCGG539350.08.794972120
AGATCTC594450.08.788862120
GATCTCG585700.08.74566120
GTGGTCG327850.08.710571120
GTGTAGA822500.08.603373120
TGGTCGC296050.08.590204120
CGGTGGT410550.08.552144120
GGTCGCC302450.08.468065120
AGTGTAG855650.08.452744120
GAGTGTA915300.08.361672120
GTAGATC811750.08.30255120
TGTAGAT848750.08.174369120
AGAGTGT991250.07.95754120
AAGAGTG1090900.07.947097120
Produced by FastQC (version 0.11.5)