FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11326412
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC136100.083.10041120
GAGCACA219000.051.992836120
AGAGCAC299800.037.875443118-119
AAGAGCA481300.024.62534120
GAAGAGC520200.023.091232120
GGAAGAG672400.017.822956118-119
TCGGAAG777750.015.712763120
CGGAAGA776950.015.514212120
ATCGGAA933800.013.105462118-119
GATCGGA956900.012.95991120
AGATCGG1025350.012.06084120
TAGATCG339700.011.582294120
CTAGATC80650.010.300001114-115
GAGATCG408700.09.98406120
ACACGTC32950.09.493598120
TTAGATC96850.08.900119120
ATAGATC210250.08.778064118-119
CAGATCG329400.08.694555120
CACGTCT33300.08.558809120
CCTAGAC7350.00302696378.511947120
Produced by FastQC (version 0.11.5)