FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17818312
Sequences flagged as poor quality0
Sequence length51-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA2538650.014.483243120
TCTCGGG35600.012.9774885120
GCGTCGG126100.011.847704120
GTATCAT1110950.09.942812120
CGTATCA1135150.09.841841120
GCCGTAT1356600.09.7744055120
CCGTATC1257900.09.534909120
TCATTAA926800.09.218789120
CATTAAA871100.09.016159120
ATCATTA1053350.08.885908120
GTCGCCG1726100.08.738748120
CGCCGTA1450500.08.707315120
TCGCCGT1621950.08.489755120
GGTCGCC1795250.08.455621120
TATCATT1182500.08.321327120
TGGTCGC1917000.08.300444120
GTGGTCG2067500.08.204085120
GGTGGTC2206400.08.095515120
TCGGTGG2563300.07.9444227120
CCTAGCT8405.35312E-47.8571191
Produced by FastQC (version 0.11.5)