FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17818312
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC266000.0130.87836120
GAGCACA462350.075.4354120
AGAGCAC666050.052.748596120
AAGAGCA981950.036.043446118-119
GAAGAGC1124350.031.296198116-117
GGAAGAG1362050.026.038765116-117
CGGAAGA1587550.022.171627114-115
TCGGAAG1669150.021.210688114-115
ATCGGAA1920700.018.40943112-113
GATCGGA2003150.017.71773112-113
AGATCGG2156000.016.37967110-111
TAGATCG603750.015.021595110-111
GAGATCG866850.013.930504110-111
CAGATCG838800.013.379304110-111
CTAGATC101800.013.086549108-109
ATAGATC345700.012.669571108-109
GTAGATC197750.011.674118108-109
AGAGATC416700.011.493235114-115
TTAGATC126900.011.216305110-111
ACAGATC403800.010.923109112-113
Produced by FastQC (version 0.11.5)