FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004831999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004831999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16067735
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC201500.075.14497120
GAGCACA319350.045.88049120
AGAGCAC427450.035.97205120
AAGAGCA640550.024.133997120
GAAGAGC723850.021.775997120
GGAAGAG885700.018.47432120
TCGGAAG1053350.015.518408118-119
CGGAAGA1064050.015.410176120
ATCGGAA1256300.013.293578120
GATCGGA1285950.012.762528116-117
AGATCGG1421850.011.95924120
TAGATCG297600.011.059464118-119
CACACGT54850.010.941656120
CTAGATC78100.010.623963112-113
CACGTCT63000.09.854677120
TTAGATC105400.09.70119118-119
ACACGTC68000.08.8094635118-119
GCTAGAT33000.08.779621120
GAGATCG540850.08.720731118-119
AAGATCG491550.08.714939120
Produced by FastQC (version 0.11.5)