FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004832002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004832002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15855473
Sequences flagged as poor quality0
Sequence length53-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC190150.060.1668933
CTTCCGA198550.058.1392021
CCGATCT194850.058.1304134
CGATCTA51150.052.4280555
TTCCGAT239350.048.0498352
CGATCTC125750.026.0010175
CGATCTT128050.024.5501165
CGATCTG115900.021.0157285
GATCTAG26550.020.5626346
GATCTAA43600.020.3646566
GCGTCGG86050.018.336609120
GATCTCA74850.016.671456
GATCTTA67200.015.3553966
GATCTAT62100.014.684326
TCTCGGG35000.013.370287120
AAAAAAA2771900.013.070785120
GATCTCT67400.012.6395126
GATCTAC47250.011.0463626
CCGTATC1200350.09.57139120
GTATCAT1068700.09.509808120
Produced by FastQC (version 0.11.5)